Labs

  • The newly organized Peptide Synthesis Core Facility (PSCF) contains a dedicated fume hood for peptide deprotection and cleavage. Its instrumentation includes a new CEM Liberty automated Microwave Multiple CHS Peptide Synthesizer, a new Waters Preparative Scale AutoPurification HPLC System for high-throughput applications, a Varian analytical scale HPLC for assessing product purity, three Savant preparative scale speed-vacs, and a new Virtis BenchTop6K freeze dryer. The facility hopes to soon purchase a compact Waters 3100 Mass Detector that is specifically designed for Waters AutoPurification HPLC system increasing productivity and the quality of our service to the UCLA community.

    This core facility was established to serve the needs of UCLA faculty performing multifaceted investigations of peptides. The facility will provide peptides that are difficult to synthesize, fold, and purify, e.g., beta-sheet peptides with multiple disulfide connectives, cyclic peptides, or amyloid peptides. In addition, the PSCF will synthesize isotope-edited versions of proteins and peptides suitable for detailed determinations of three-dimensional molecular structure by NMR spectroscopy or X-ray diffraction.

  • The UCLA Pasarow Mass Spectrometry Laboratory is an intellectually vibrant group dedicated to the advancement of the science and training of students through innovative research and educational endeavors. As of January 2009 there are eight full-time members including two faculty, two senior technicians, two post docs, one Ph.D. students and one of undergraduate, in addition to numerous other UCLA faculty, staff and students who make frequent use of the facility and the in-house expertise. Our publication record is easily accessed via the web using Faull, KF and Whitelegge, JP as search descriptors. NSF and NIH Shared Instrument Grants over the past few years had led to the acquisition of several new instruments that complete the suite of available instrumentation. The availability of electron impact (EI), chemical (CI), atmospheric chemical ionization (APCI) and electrospray (ESI) ionization methods together in one facility is a feature of this Laboratory that is vital to the variety of chemical and biochemical research at UCLA. The Laboratory is also equipped a range of modern equipment necessary for the successful operation of a wet biochemical laboratory, including gas (capillary) and liquid chromatography (analytical, micro- and nano-bore) capability and gel electrophoresis.

  • The Molecular Screening Shared Resource (MSSR) offers a comprehensive range of leading-edge high throughput screening (HTS) services including drug discovery, chemical and functional genomics, and novel methods for nano and environmental toxicology. The MSSR is an open access environment with investigators from UCLA as well as from the entire globe. Industrial clients are equally welcome as are non-profit entities.

  • The High-Throughput Clinical Proteomics Core (HTCP) is a mass spectrometry core facility designed to profile large numbers of clinical samples for changes in protein content. The core uses small amounts (micrograms) of protein from samples such as urine, plasma, cerebrospinal fluid, and cell lysates and fractionates them using solid-phase extraction onto magnetic beads. The different subsets of bound proteins on each bead type are examined by high-resolution MALDI-TOF mass spectrometry. The relative amount and precise intact masses are measured and analyzed for disease-specific changes. The MALDI-TOF instrument measures the intact masses of proteins and peptides at sub-femtomolar sensitivities with an accuracy of 5-50 ppm (>10,000 resolution FWHM) and maximum sensitivity below 20 kDa. Once specific proteins are determined to be important for particular patient group fractionation, they are isolated by HPLC, digested with trypsin, and identified by tandem MS/MS fragmentation.

  • This facility is prepared to give instructions in microscopic techniques and assist you in preparing tissue specimens for light microscopic observation. Histological procedures available include some immunocytochemistry staining, special stains, paraffin sectioning, slide preparation for in situ hybridization, cryostat sectioning, plastic embedding and sectioning. The laboratory also provides staining setups, a cryostat, microtomes, and a Nikon photomicroscope for use by trained personnel.

    Histological procedures available include some immunocytochemistry staining, special stains, paraffin sectioning, slide preparation for in situ hybridization, cryostat sectioning, plastic embedding and sectioning.

  • The Biological Samples Processing Core (BSPC) offers DNA extractions from whole blood and other tissue types utilizing the Autopure LS nucleic acid purification instrument from Gentra Systems. Extracted DNA can be returned immediately or stored indefinitely at the BSPC in a -20°C freezer, for future use. The core also aliquots DNA at desired concentrations. All samples are logged in the BSPC database and are bar-coded with their sample number and customer's identification code.

  • The UCLA Neuroscience Genomics Core (UNGC) performs sequencing, genotyping and gene expression resource for academic labs in the Southern California region and beyond, the UNGC has been created to offer academic researchers the highest quality custom snp genotyping, whole genome genotyping, whole genome gene expression screens and sequencing services at the lowest possible cost.

  • The Technology Center for Genomics & Bioinformatics (TCGB) is a fully automated, high-throughput genomic facility equipped with all major next generation sequencing and microarray platforms. TCGB provides next generation sequencing and microarray-based genomic services, including Whole genome sequencing, Whole Exon Sequencing, Whole Transcriptome Sequencind (RNA-Seq), Small RNA Sequencing (miRNA-Seq), Targeted Resequencing, Methylation Sequencing (Methy-Seq), SNP Genotyping, in a cost-effective and timely manner to serve basic science, translational, and clinical researchers

  • The UCLA DNA Microarray Core Facility offers data analysis services for fee for your microarray experiments. Data Analysis gives users knowledge typically used to identify important genes with characteristic expression patterns from their microarray experiments.

    For gene expression arrays, we offer differential two group and multigroup gene expression analysis, and identify statistically,
    differentially expressed genes between the different conditions the users are comparing, using statistical criteria appropriate for the
    dataset size.