Labs

  • The newly organized Peptide Synthesis Core Facility (PSCF) contains a dedicated fume hood for peptide deprotection and cleavage. Its instrumentation includes a new CEM Liberty automated Microwave Multiple CHS Peptide Synthesizer, a new Waters Preparative Scale AutoPurification HPLC System for high-throughput applications, a Varian analytical scale HPLC for assessing product purity, three Savant preparative scale speed-vacs, and a new Virtis BenchTop6K freeze dryer. The facility hopes to soon purchase a compact Waters 3100 Mass Detector that is specifically designed for Waters AutoPurification HPLC system increasing productivity and the quality of our service to the UCLA community.

    This core facility was established to serve the needs of UCLA faculty performing multifaceted investigations of peptides. The facility will provide peptides that are difficult to synthesize, fold, and purify, e.g., beta-sheet peptides with multiple disulfide connectives, cyclic peptides, or amyloid peptides. In addition, the PSCF will synthesize isotope-edited versions of proteins and peptides suitable for detailed determinations of three-dimensional molecular structure by NMR spectroscopy or X-ray diffraction.

  • The UCLA Pasarow Mass Spectrometry Laboratory is an intellectually vibrant group dedicated to the advancement of the science and training of students through innovative research and educational endeavors. As of January 2009 there are eight full-time members including two faculty, two senior technicians, two post docs, one Ph.D. students and one of undergraduate, in addition to numerous other UCLA faculty, staff and students who make frequent use of the facility and the in-house expertise. Our publication record is easily accessed via the web using Faull, KF and Whitelegge, JP as search descriptors. NSF and NIH Shared Instrument Grants over the past few years had led to the acquisition of several new instruments that complete the suite of available instrumentation. The availability of electron impact (EI), chemical (CI), atmospheric chemical ionization (APCI) and electrospray (ESI) ionization methods together in one facility is a feature of this Laboratory that is vital to the variety of chemical and biochemical research at UCLA. The Laboratory is also equipped a range of modern equipment necessary for the successful operation of a wet biochemical laboratory, including gas (capillary) and liquid chromatography (analytical, micro- and nano-bore) capability and gel electrophoresis.

  • The Molecular Screening Shared Resource (MSSR) offers a comprehensive range of leading-edge high throughput screening (HTS) services including drug discovery, chemical and functional genomics, and novel methods for nano and environmental toxicology. The MSSR is an open access environment with investigators from UCLA as well as from the entire globe. Industrial clients are equally welcome as are non-profit entities.

  • Provides consulting including grant preparation and timely help to researchers in the health sciences and other areas. Experineced in a wide range of applications, including clinical trials, laboratory studies, surveys, epidemilogical studies, and many others.

  • The Semel Institute Biostatistics Core (SIStat) is a team of expert biostatisticians, methodologists and research support staff. The mission of the Core is to foster research productivity and quality by helping faculty design sound research projects, obtain independent funding, and analyze data correctly. Grant development services such as design and statistical consultation, power analysis, and analyses of pilot data are available to Semel Institute faculty without charge.

  • As with other areas of biomedical science, the post-genome era raises the prospect of transformational advances in neuroscience research. Neuroscience faces special challenges, however, in analysis, interpretation, and management of the vast quantities of information generated by genetic and genomic technologies. The phenotypic and organizational complexity of the nervous system calls for distinct analytical and informatics strategies and expertise. The ICNN will provide advanced analysis and informatics support to a highly interactive user group consisting of neuroscientists at UCLA, who are conducting basic, clinical, and translational research. These investigators have access to excellent facilities for genetics and genomics experimentation; the lack of corresponding resources in analysis and informatics constitutes a bottleneck in their research. ICNN faculty experts in statistical genetics, gene expression analysis, and bioinformatics will oversee the activities of highly-trained staff members who will accomplish three goals:

    Providing expert consultation and analyses for neurogenetics and neurogenomics projects;

    Developing and maintaining a shared computing resource that will include a computational cluster for computation-intensive analyses, web-servers and state of the art software tools for a wide range of applications (including user-friendly versions of public databases, as well as workstations on which ICNN users will be trained to employ these tools;

    Providing hands-on training in analysis and informatics to the users.

  • The primary objective of DOMStat is to provide state-of-the-art study design and statistical analysis collaboration to investigators in the Department of Medicine as well as faculty from across UCLA. DOMStat offers faculty and staff level statistical collaboration for grant proposals, data analysis, manuscript preparation, and other research activities requiring statistical input. We have expertise in clinical trials, high-throughput molecular analysis, observational studies, and large scale secondary data analyses.

  • The objective of the UCLA Vector Core is to promote and facilitate basic and translational research by providing investigators with access to vector technologies that enable efficient gene transfer to mammalian cells in culture and in vivo. To this end, the Vector Core is currently:

    Serving as an educational and advisory resource for UCLA researchers who may have had limited experience with virus-derived gene transfer vector technologies, but who wish to utilize such technologies for efficient functional expression of genetic sequences of interest in mammalian cell culture and in animal models in vivo

    Providing, at minimal cost, various pre-made retroviral, lentiviral, and adenoviral vector stocks expressing standard marker genes to utilize in preliminary experiments, as well as a library of available vectors expressing a variety of mammalian genes and corresponding inhibitory sequences

    Designing and producing custom viral vectors that contain a specific transgene of interest (including wild type and mutant cDNAs with or without epitope tags, dominant-negative expression constructs, antisense mRNAs, siRNAs, etc.) for individual researchers. A bank of lentiviral vectors including transfer vectors, vectors expressing marking genes and customized vectors is available.

    The Vector Core assists investigators from academia, and private companies.

  • Provides testing services, blood and blood cell products and other specialized services to research labs working with HIV and related viruses.