The UCSD Screening Core provides infrastructure and consulting expertise for the development and implementation of screening assays in small, medium and high-throughput scale.
The mission of the UCLA X-ray Crystallography Core Facility is to provide state-of-the-art resources to researchers at UCLA., enabling the detailed 3-D analysis of biological macromolecules that play essential roles in human health. The facility operates as a full service core that not only offers access to sophisticated equipment and technologies, but also offers advice and technical assitance in sample preparation, data collection, processing, atomic refinement and modeling.
The purpose of the X-ray Crystallography Core Facility is to provide equipment, training, assistance, and technological innovation for determining 3D atomic structures of proteins and other macromolecules.
The X-ray Facility conducts original research in developing and improving crystallographic tools, and helps scientists determine atomic structures. Each structure determined using the Facility not only yields insight into biochemical pathways, but also expands databases used for improving algorithms for fold assignment, structure verification, atomic refinement, potential energy functions, and analysis of protein-protein interactions.
The Molecular Screening Shared Resource (MSSR) offers a comprehensive range of leading-edge high throughput screening (HTS) services including drug discovery, chemical and functional genomics, and novel methods for nano and environmental toxicology. The MSSR is an open access environment with investigators from UCLA as well as from the entire globe. Industrial clients are equally welcome as are non-profit entities.
The UCLA Crystallization Facility provides state-of-the-art, high-throughput, crystallization services to all institutions. The full-service core offers access to sophisticated equipment and technologies, and technical assistance in sample preparation. The facility will provide screen preparation, plate setup, automated image acquisition and analysis, and optimization design. Our high-throughput set up can produce 18,000 experiments in a single day.
The objective of the UCLA Vector Core is to promote and facilitate basic and translational research by providing investigators with access to vector technologies that enable efficient gene transfer to mammalian cells in culture and in vivo. To this end, the Vector Core is currently:
Serving as an educational and advisory resource for UCLA researchers who may have had limited experience with virus-derived gene transfer vector technologies, but who wish to utilize such technologies for efficient functional expression of genetic sequences of interest in mammalian cell culture and in animal models in vivo
Providing, at minimal cost, various pre-made retroviral, lentiviral, and adenoviral vector stocks expressing standard marker genes to utilize in preliminary experiments, as well as a library of available vectors expressing a variety of mammalian genes and corresponding inhibitory sequences
Designing and producing custom viral vectors that contain a specific transgene of interest (including wild type and mutant cDNAs with or without epitope tags, dominant-negative expression constructs, antisense mRNAs, siRNAs, etc.) for individual researchers. A bank of lentiviral vectors including transfer vectors, vectors expressing marking genes and customized vectors is available.
The Vector Core assists investigators from academia, and private companies.
The Mitochondria and Metabolism Core at UCLA provides the scientific community with the necessary technical and conceptual resources for studying mitochondrial function. We provide access to Seahorse XF96 Extracellular Flux Analyzers and an Operetta High Content Imager, which users can schedule after training. Additionally, The Mitochondria Core provides a number of full service options for the design, performance, analysis and interpretation of fundamental mitochondrial assays.
Orian Shirihai, MD PhD
The purpose of the newly finished Zebrafish Core is to facilitate the use and quick access of common mutations, genetically engineered transgenic zebrafish and routine techniques of zebrafish manipulation by investigators at UCLA. The core will provide the UCLA research community with four major categories of service.
1. Space for housing zebrafish and performing larger scale genetic or chemical genomic screens
2. Assistance with experiments using zebrafish
3. Generation of transgenic zebrafish
4. Cryostorage of zebrafish sperm and re-derivation of live fish