The UCSD Screening Core provides infrastructure and consulting expertise for the development and implementation of screening assays in small, medium and high-throughput scale.
The MMSF at UC San Diego provide routine MS analysis on various types of samples including small organic molecules, peptides, natural products, organometallic compounds, intact proteins, polymers, oligonucleotides, antibodies, and antibody-drug conjugates.
Yongxuan Su, Ph.D.
Access to NMR technology for both UCSD and external users. Users can collect their own data, facility staff can do it all for you, or collaborative projects can be developed. The facility houses two 600 MHz Bruker Avance III spectrometers with cryogenically cooled probes for enhanced sensitivity. For samples whose concentration is limited by the amount of material available, or by solubility, our 1.7 mm microcoil cryoprobe requires just 40 μl of solution. We provide training in instrument operation and can offer advice for data analysis or project feasibility.
The UCLA MIC Mass Spectrometry Laboratory provides a wide range of sample characterization techniques for UCLA researchers. Ionization methods currently available include electron impact (EI), chemical (CI), matrix assisted laser desorption (MALDI), electrospray ionization (ESI), and atmospheric pressure chemical ionization (APCI). Analyzers currently available include time of flight, quadrupole, ion trap, ion cyclotron resonance analyzers, and several tandem combinations of mass analyzers.
The UCLA Pasarow Mass Spectrometry Laboratory is an intellectually vibrant group dedicated to the advancement of the science and training of students through innovative research and educational endeavors. As of January 2009 there are eight full-time members including two faculty, two senior technicians, two post docs, one Ph.D. students and one of undergraduate, in addition to numerous other UCLA faculty, staff and students who make frequent use of the facility and the in-house expertise. Our publication record is easily accessed via the web using Faull, KF and Whitelegge, JP as search descriptors. NSF and NIH Shared Instrument Grants over the past few years had led to the acquisition of several new instruments that complete the suite of available instrumentation. The availability of electron impact (EI), chemical (CI), atmospheric chemical ionization (APCI) and electrospray (ESI) ionization methods together in one facility is a feature of this Laboratory that is vital to the variety of chemical and biochemical research at UCLA. The Laboratory is also equipped a range of modern equipment necessary for the successful operation of a wet biochemical laboratory, including gas (capillary) and liquid chromatography (analytical, micro- and nano-bore) capability and gel electrophoresis.
The Molecular Screening Shared Resource (MSSR) offers a comprehensive range of leading-edge high throughput screening (HTS) services including drug discovery, chemical and functional genomics, and novel methods for nano and environmental toxicology. The MSSR is an open access environment with investigators from UCLA as well as from the entire globe. Industrial clients are equally welcome as are non-profit entities.
The Magnetic Resonance Laboratory consists of six nuclear magnetic resonance spectrometers (NMR) and one electron paramagnetic resonance spectrometer (EPR). Users of the facility usually run their own spectra. For routine NMR spectra, an automatic sample changer is available on one of the spectrometers. All users must be trained by the facility staff. The laboratory is a part of the UCLA Molecular Instrumentation Center (MIC), which is a campus wide facility open to the UCLA community. Further information can be obtained at the website below.
The Mitochondria and Metabolism Core at UCLA provides the scientific community with the necessary technical and conceptual resources for studying mitochondrial function. We provide access to Seahorse XF96 Extracellular Flux Analyzers and an Operetta High Content Imager, which users can schedule after training. Additionally, The Mitochondria Core provides a number of full service options for the design, performance, analysis and interpretation of fundamental mitochondrial assays.
Orian Shirihai, MD PhD