Pasarow Mass Spectrometry Laboratory

The UCLA Pasarow Mass Spectrometry Laboratory is an intellectually vibrant group dedicated to the advancement of the science and training of students through innovative research and educational endeavors. As of January 2009 there are eight full-time members including two faculty, two senior technicians, two post docs, one Ph.D. students and one of undergraduate, in addition to numerous other UCLA faculty, staff and students who make frequent use of the facility and the in-house expertise. Our publication record is easily accessed via the web using Faull, KF and Whitelegge, JP as search descriptors. NSF and NIH Shared Instrument Grants over the past few years had led to the acquisition of several new instruments that complete the suite of available instrumentation. The availability of electron impact (EI), chemical (CI), atmospheric chemical ionization (APCI) and electrospray (ESI) ionization methods together in one facility is a feature of this Laboratory that is vital to the variety of chemical and biochemical research at UCLA. The Laboratory is also equipped a range of modern equipment necessary for the successful operation of a wet biochemical laboratory, including gas (capillary) and liquid chromatography (analytical, micro- and nano-bore) capability and gel electrophoresis.

The PMSL currently houses five mass spectrometers, used for the structural determination and quantification of a wide variety of organic and inorganic compounds.

Kym Faull
faull@chem.ucla.edu
310.206.7881

Julian Whitelegge
jpw@chem.ucla.edu

Farbod Fazlollahi
farbodfazlollahi@gmail.com

Joseph Capri
capri@chem.ucla.edu

ServiceUCLA CostJCCC/CFAR CostExternal Cost
DONE BY PMSL STAFF$60.00  
GC/MS derivatization, instrument set up and running of samples (per sample)$100.00  
GC/MS derivatization, instrument set up and running of samples (per hour)$1,000.00  
GC/MS derivatization, instrument set up and running of samples (per day)$120.00  
GC/MS derivatization, instrument set up and running of samples -rush (per sample)$200.00  
GC/MS derivatization, instrument set up and running of samples -rush (per hour)$2,000.00  
GC/MS derivatization, instrument set up and running of samples -rush (per day)$60.00  
LC/MS, sample preparation, instrument set up and running of samples (per sample)$100.00  
LC/MS, sample preparation, instrument set up and running of samples (per hour)$1,000.00  
LC/MS, sample preparation, instrument set up and running of samples (per day)$120.00  
LC/MS, sample preparation, instrument set up and running of samples-rush (per sample)$200.00  
LC/MS, sample preparation, instrument set up and running of samples-rush (per hour)$2,000.00  
LC/MS, sample preparation, instrument set up and running of samples-rush (per day)$100.00  
Data analysis$1,000.00  
GC/MS (per hour)$200.00  
GC/MS (per day)$2,000.00  
GC/MS- rush (per hour)$100.00  
GC/MS- rush (per day)$1,000.00  
LC/MS including bottom-up proteomics (per hour)$200.00  
LC/MS including bottom-up proteomics (per day)$2,000.00  
LC/MS including bottom-up proteomics-rush (per hour)$100.00  
LC/MS including bottom-up proteomics-rush (per day)$500.00  
DONE BY SELF (Instrument time)$100.00  
GC/MS (per hour)$500.00  
GC/MS (per day)$100.00  
LC/MS Sciex (per hour)$500.00  
LC/MS Sciex (per day)$100.00  
LC/MS Agilent 6460 (per hour)$500.00  
LC/MS Agilent 6460 (per day)   
LC/MS Agilent QTOF 6550 (per hour)   
LC/MS Agilent QTOF 6550 (per day)   
SPECIFIC ANALYSES DONE BY PMSL STAFF   
Protein identification (bottom-up proteomics on gel bands with data analysis by Mascot)   
Protein identification (plasma/tissue using filter-assisted sample preparation with stage-tip SCX and multiple fractions analyzed, typically 10; Mascot, Discoverer, others)   
Protein post-translational modification identification (as above with enrichment of phosphopeptides etc)   
Quantitative proteomics (label-free using Progenesis LCMS for aligning and comparing chromatograms)    
Quantitative proteomics (dimethyl labeling; triplex stable isotope experiment with MaxQuant and Discoverer)   
Intact protein mass measurement and profiling – low-resolution ESI typically LC-MS.    
Reverse-phase LC-MS with fraction collection (LC-MS+). Fractions reserved for top-down high-resolution FT-MS and other experiments.   
Intact protein mass measurement and profiling – high-resolution ESI on Agilent Q-tof.    
Intact protein mass measurement and profiling - high-resolution ESI on FT-MS (Orbitrap, 7T LTQ-FT, 15T Bruker).   
Top-down mass spectrometry - high-resolution ESI on FT-MS (Orbitrap, 7T LTQ-FT, 15T Bruker) with collisionally activated or electron capture/transfer dissociation (CAD and ECD/ETD; Prosight PC 3.0).   
Intact protein quantification – EIC method (low or high resolution).   
Protein quantification using stable-isotope labeled peptides and QQQ MS/MS-MRMs (Agilent Masshunter B.05; Skyline)   
Native protein mass spectrometry – static nanospray on Orbitrap/LTQFT to 4000 m/z; syringe pump infusion Qtof to 10,000 m/z   
Immunocapture-MS. Protein capture from complex fluids using immobilized antibody columns.   
Pharmacokinetics; measurement of small molecules and peptides in complex matrices(tissue homogenates, plasma, urine, etc)   
Metabolomics screeening of plasma, CSF, seminal fluid, urine, saliva, tissue extracts, cell culture extracts and tears by GC/MS   
Metabolomics screeening of plasma, CSF, seminal fluid, urine, saliva, tissue extracts, cell culture extracts and tears by GC/MS