X-Ray Crystallography (DOE)

The mission of the UCLA X-ray Crystallography Core Facility is to provide state-of-the-art resources to researchers at UCLA., enabling the detailed 3-D analysis of biological macromolecules that play essential roles in human health. The facility operates as a full service core that not only offers access to sophisticated equipment and technologies, but also offers advice and technical assitance in sample preparation, data collection, processing, atomic refinement and modeling.

The purpose of the X-ray Crystallography Core Facility is to provide equipment, training, assistance, and technological innovation for determining 3D atomic structures of proteins and other macromolecules.

The X-ray Facility conducts original research in developing and improving crystallographic tools, and helps scientists determine atomic structures. Each structure determined using the Facility not only yields insight into biochemical pathways, but also expands databases used for improving algorithms for fold assignment, structure verification, atomic refinement, potential energy functions, and analysis of protein-protein interactions.

Todd Yeates
yeates@mbi.ucla.edu

Dulio Cascio
cascio@mbi.ucla.edu

ServiceUCLA CostJCCC/CFAR CostExternal Cost
Providing experimental and computational facilities for X-ray based structure analysis and   refinement, and for supporting structure determination by crystallography, and computational methods.   
Acquisition of X-ray diffraction data using in-house high brilliance generators.   
Acquisition of Multi-wavelength Anomalous Dispersion data using synchrotron radiation   
X-ray diffraction data processing and manipulation.   
Assistance with the macromolecular model building and structure refinement processes.   
Guidance in the interpretation of the resulting three-dimensional macromolecular structures.   
Web-based services that include tutorials and software tools to assist users.   
Workshops and informal classes introducing the fundamentals of single crystal X-ray diffraction work to potential users.   
Micro crystallography: rapid screening of micro-crystals with the UV microscope.  Manipulation and dissection of micro-crystals with a micro-manipulator.   
*fee dependent on "units" or level of usage (1-6). 1 unit=$1800 per quarter