Zebrafish

The purpose of the newly finished Zebrafish Core is to facilitate the use and quick access of common mutations, genetically engineered transgenic zebrafish and routine techniques of zebrafish manipulation by investigators at UCLA. The core will provide the UCLA research community with four major categories of service.

1. Space for housing zebrafish and performing larger scale genetic or chemical genomic screens
2. Assistance with experiments using zebrafish
3. Generation of transgenic zebrafish
4. Cryostorage of zebrafish sperm and re-derivation of live fish

Shuo Lin, PhD
shuolin@ucla.edu

ServiceUCLA CostJCCC/CFAR CostExternal Cost
Adult animals, wild type, 10 pairs$87.00 $414.00
Embryos, wild type, 600$87.00 $414.00
Embryos, transgenic, 600 (Not allowed to be raised and distributed)$174.00 $828.00
Plasmid construct injection for a transgenic line$696.00 $3,312.00
Preparation of regular plasmid construct for injection$261.00 $1,242.00
Screening for transgenic (regular plasmid construct) F1 embryos$435.00 $2,070.00
BAC / PAC construct injection for a transgenic line$1,044.00 $4,968.00
Preparation of BAC / PAC construct for injection$522.00 $2,484.00
Screening for transgenic (BAC / PAC) F1 embryos$696.00 $3,312.00
Injection training (3 times)$348.00 $1,656.00
Construction of a DNA construct for injection (range – actual cost depending upon specific requirements)$261.00 $1,242.00
Morpholino injection (3 times)$522.00 $2,484.00
Freezing sperms of 10 male animals (minimum)$522.00 $2,484.00
Training for freezing sperms (3 times)$348.00 $1,656.00
Housing (Monthly rate per rack) $348.00